Interaction between FUS/TLS and PRMT1 in mammalian cells.

<p>(A) Co-immunoprecipitation assay between HA-tagged FUS/TLS and Myc-tagged PRMT1 protein. HEK293 cells were transiently transfected with the indicated plasmids. Cell lysates were immunoprecipitated with anti-HA antibody, and then immunoprecipitates were immunoblotted with anti-Myc antibody (upper panel). Cell lysates were immunoblotted with anti-HA and anti-Myc antibody (lower panel). IB, Immunoblot; IP, Immunoprecipitation. (B) Co-immunoprecipitation assay between endogenous FUS/TLS and PRMT1 protein. The lysates of HEK293 cells were immunoprecipitated with anti-FUS/TLS antibody or control mouse IgG. Then immunoprecipitates were immunoblotted with anti-PRMT1 (upper panel) or anti-FUS/TLS antibody (lower panel). IgG HC, Immunoglobulin heavy chain. IgG LC, Immunoglobulin light chain. (C) HEK293 and SH-SY5Y cells were co-stained with anti-FUS/TLS and anti-PRMT1 antibody, and followed by the incubation with Alexa488 and Alexa568 conjugated secondary antibody (Invitrogen). Samples were then stained with DAPI. Images were obtained using fluorescence microscopy (Nikon, E600). Bars, 20 µm.