TMPRSS6 downregulation by inflammation is mediated by p-Stat5.

<p>(A) Liver nucleic lysates from mice were analyzed by Western blot for p-Stat5 relative to TBP. Values shown are means of expression values divided by a calibrator quantity (the mean value of expression for the baseline group)+/− SEM. Means in baseline and treated groups were compared by student t tests. (B) Hepatic nuclear extracts from mice at Baseline or 16 hrs after injection with LPS (n = 4 per group) were subjected to EMSA using a Stat5 binding sequence from the mouse <i>Tmprss6</i> promoter (left panel). Nucleic acids and bound proteins were transferred on membrane and analyzed by Western blot for Stat5. Stat5 shift was quantified using Li-Cor software. (C) Livers from Baseline mice (n = 5) were subjected to ChIP with Stat5 antibody and normal IgG antibody. Recovered DNA was analyzed for the presence of TMPRSS6 promoter. P values p<0.05 were considered statistically significant. Significances are: *p<0.05; ***p<0.005.