Production of IL-1β <i>in vivo</i> is dependent on MYD88 and NLRP3.

(A-C) Indicated cytokine levels were examined in whole lung homogenates on day 9 post-PR8, day 2 post-S.p. or day 2 post-coinfection. (D-I). Weight loss and mortality in mice infected with PR8 alone, S.p. alone, or PR8-S.p. coinfection. (A-C) Data are representative of two experiments, n = 5–7 mice per group per experiment. One-way ANOVA using Tukey’s post hoc analysis was used for statistical comparison (Mean +/- SEM). (D-I) Data are combined from 2–3 experiments, total n is indicated. Two-way ANOVA using Tukey’s post hoc analysis was used for statistical comparison for weight loss (Mean +/- SEM) and Kaplan-Meier Survival Plot and LogRank Test for survival data. ns: not significant, p values: <0.05 (*), <0.01 (**), <0.001 (***).