Cytokine concentration in BAL is altered by exercise and obesity.

<p>Mice from each treatment group were infected with A/PR/8/34, and then euthanized at the indicated time points. BAL cytokine concentration (pg/mL) was assessed by a multiplex assay on the Luminex platform. The acronym ‘n.d.’ denotes that the protein was not-detectable in the non-infected group. Two-way ANOVA was used to test for statistical significance; d X e* indicates a significant (<i>p</i> < 0.05) interaction between diet and exercise (d X e+; <i>p</i> < 0.1). A main effect of exercise is indicated by e* (<i>p</i> < 0.05) (e+; <i>p</i> < 0.1). A main effect of diet is signified by d* (<i>p</i> < 0.05) (d+; <i>p</i> < 0.1). Sample size (n) equals 8–13 mice per infected group; for non-infected groups n = 1–3. Data shown as mean ± SEM. Results are representative of two separate experiments.