Additional file 6: of A central-acting connexin inhibitor, INI-0602, prevents high-fat diet-induced feeding pattern disturbances and obesity in mice

Figure S6. INI-0602 given after initiating HFD feeding did not restore locomotor activity patterns in mice. Locomotor activity results for the same mice that were analyzed in Fig. 6, treated with vehicle (V, n = 6, white squares with dashed line) or INI-0602 (Tx, n = 6, black circles with solid line). White and black bars on the X-axis correspond to the light and dark cycles, respectively. Vertical dashed line indicates the switch from NC (Pre) to HFD and the initiation of IP injections (Post). (a–b) Hourly locomotor activity pattern over the course of the study in (a) the vehicle group and (b) the treated group. (c–e) Locomotor activity during (c) the light cycle, (d) the dark cycle, and (e) each 24-h period. (f–h) Cosinor analyses of the locomotor activity rhythms, including the (f) period length, (g) amplitude, and (h) nadir of the CT. (i) Actograms of two representative mice in each group. White and black bars above the traces correspond to the light and dark cycles, respectively. Data are the means ± s.e.m. Statistical significance was determined with the Student’s t-test for comparisons between groups at each time point, in c–e. Significant differences were determined with a one-way ANOVA with post-hoc Student’s t-test and the Bonferroni correction for comparisons among groups, in f-h. Abbreviations: HFD, high-fat diet; A.U., arbitrary unit; CT, circadian time. (TIF 1284 kb