Additional file 1: Figure S1. of Resolvin RvD2 reduces hypothalamic inflammation and rescues mice from diet-induced obesity

Six-week-old, male Swiss mice were stereotaxically instrumented in a Stoelting stereotaxic apparatus to receive a cannula placed in the lateral hypothalamic ventricle, using the following stereotaxic coordinates: anteroposterior 0.34 mm, lateral 1.0 mm, and dorsoventral 2.2 mm. The correct position of the cannula was tested 5 days after surgery by evaluation of the thirst response elicited by intracerebroventricular (icv) angiotensin II (106 M). After 1 week, icv-cannulated mice were transferred to the high-fat diet and treated once a day for 3 days with 2 μL of saline (CTR) or 2 μL of DHA (5, 10 or 20 ng). Caloric intake and body mass were determined during the experimental period. At the end of the experimental period, the hypothalamus was obtained for real-time PCR quantitative determination of the transcripts encoding for PLA2 (A), 15-LOX (B), 5-LOX (C), GPR18 (D), IL6 (E) and IL10 (F). The methods for hypothalamic extraction and real-time PCR are described in the Methods section of the paper. (PDF 768 kb