Additional file 5: of A central-acting connexin inhibitor, INI-0602, prevents high-fat diet-induced feeding pattern disturbances and obesity in mice

Figure S5. INI-0602 did not block HFD-induced microglial activation or inflammatory cytokine expression in the hypothalamus. Mice were fed HFD for 1 or 3 days, as indicated, and they received daily intraperitoneal injections of vehicle (V, n = 3-4, white symbols) or INI-0602 (Tx, n = 4, black symbols). (a-f) Histological analyses tissue sections of the ARC of the hypothalamus. (a and d) Representative photomicrographs of ARC sections show microglia stained with anti-Iba-1 (red) and nuclei stained with DAPI (blue). Quantifications show (b and e) Iba-1 (+) cell numbers, and (c and f) Iba-1 (+) areas. Four coronal sections were analyzed per mouse. # inidicates the section number, from rostral to caudal. (g) Quantitative PCR results show hypothalamic gene expression levels measured in another cohort of mice that underwent the same treatments. (h) The proposed point of action of INI-0602 (red) in the context of HFD feeding. Data are the means ± s.e.m. Statistical significance was determined with the Student’s t-test for comparisons between groups at each time point; *P < 0.05. Abbreviations: HFD, high-fat diet; A.U., arbitrary units. (TIF 5372 kb