Additional file 1: Figure S1. of Obesity and neuroinflammatory phenotype in mice lacking endothelial megalin

EMD mice showed exacerbated hypothalamic inflammation. (A) Higher expression of Iba-1 was observed in arcuate hypothalamic nucleus in EMD mice compared to control mice. (B) Quantitative analysis of the percentage area covered by Iba-1 immunoreactivity was performed in this brain area. (C) Higher expression of GFAP was observed in arcuate hypothalamic nucleus in EMD mice compared to control mice. (D) Quantitative analysis of the percentage area covered by GFAP immunoreactivity was performed in this brain area. Arc arcuate hypothalamic nucleus. Data are presented as mean ± SEM. ** P < 0.01; Student’s t test. Figure S2. Hippocampal NPY and leptin expression in EMD mice. (A) mRNA expression of NPY was unchanged in hippocampal samples from EMD mice compared to control mice. (B) Protein levels of leptin were unaffected in EMD compared to control mice. Data are given as mean ± SEM. Figure S3. Neurogenesis is reduced in EMD mice. (A) Representative photomicrographs of DCX (red) and DAPI nuclei (blue) staining in the hippocampal granule cell layer of EMD (n = 8), and control (n = 7) mice. Scalebar = 20 μm. (B) Number of DCX+ cells in the DG of hippocampus is reduced in EMD mice compared to control mice. (C) Fluorescent immunostaining for PSA-NCAM mainly localized in the middle and inner cell layers of the dentate gyrus in EMD (n = 8) and control (n = 7) mice. Scalebar = 20 μm. (D) PSA-NCAM+ cell number is reduced in the DG of hippocampus in EMD mice compared to control mice. Data are presented as mean ± SEM. DG dentate gyrus. *P < 0.05, **P < 0.01; Student’s t test. Related to Fig. 3. Table S1. primers used for RT-PCR. Related to Fig. 3. (DOCX 844 kb