Additional file 3: of A central-acting connexin inhibitor, INI-0602, prevents high-fat diet-induced feeding pattern disturbances and obesity in mice

Figure S3. Diet switch from NC to HFD acutely affected locomotor activity patterns in mice. Locomotor activity data for the same mice that were analyzed in Fig. 2. White and black bars on the X-axis correspond to the light and dark cycles, respectively. Vertical dashed line indicates the switch from NC (Pre) to HFD and the initiation of IP injections (Post). (a) Hourly locomotor activity over the course of the study. (b–d) Locomotor activity during (b) the light cycle, (c) the dark cycle, and (d) each 24-h period. (e-g) Cosinor analyses of the locomotor activity rhythms, including the (e) period length, (f) amplitude, and (g) nadir of the CT. (h) Actograms of two representative mice in each group. White and black bars above the traces correspond to the light and dark cycles, respectively. Data are the means ± s.e.m. Statistical significance was determined with the Student’s paired t-test, evaluated at each time point, for comparisons to the day before the diet switch (Pre in b–d, and day 0 in h). Significant differences were determined with a Student’s paired t-test for comparing data taken before and after the diet switch, in e-g. *P < 0.05. Abbreviations: A.U., arbitrary unit; CT, circadian time. (TIF 737 kb