Additional file 1: Figure S1. of Antibiotic perturbation of the murine gut microbiome enhances the adiposity, insulin resistance, and liver disease associated with high-fat diet

STAT did not change energy balance, or bone morphometrics. STAT (3 male, 3 female) and control (3 male, 3 female) mice were housed individually in metabolic cages for 5 days. A Gross caloric intake. Food consumed was measured daily by weight. Calories per gram from the chow package insert were used to calculate calories consumed. Each point represents a single 24 h of data. B Gross fecal calories. All fecal pellets were harvested for each mouse each day, and homogenized to create a daily fecal pellet for each mouse. Total dry fecal volume per day combined with bomb calorimeter values for the daily pellets were used to determine total calories out. C Net daily calories, calculated as (calories in (from A) – calories out (from B)). D Proportion of daily caloric intake retained (Net daily calories (from C) divided by gross caloric intake (from A)). Data points are the mean of duplicate determinations (Kruskal–Wallis test; *p <0.05). DEXA bone measurements for all four groups: E mineral density, F mineral content, and G area. Arrow indicates start of the HFD. p values calculated from individual mouse data (Mann–Whitney U test; *p <0.05; **p <0.01; ***p