Elevated frequencies of CD4⁺CD25⁺FoxP3⁺CD127^lo Treg in PB, BM and involved lymphatic tissues in patients with B-cell NHL.

<p>(A) Dot plots were gated on CD4⁺ T cells (based on forward and side scatter and CD4 staining). The number in the dot plot represents the percentage of gated cells expressing the relevant marker. (B) Frequencies of CD4⁺CD25⁺FoxP3⁺CD127^lo Treg in PB, BM and involved lymphatic tissues from patients with B-cell NHL and HVs were quantified using flow cytometric analysis. (C) Frequencies of CD4⁺CD25⁺FoxP3⁺CD127^lo Treg in PB, BM and involved lymphatic tissues from the same patients with B-cell NHL were measured as described earlier. (D) Frequencies of CD4⁺CD25⁺FoxP3⁺CD127^lo Treg in PB, BM and involved lymphatic tissues from indolent B-cell NHL patients and aggressive B-cell NHL patients were measured as described earlier. (E) Frequencies of CD4⁺CD25⁺FoxP3⁺CD127^lo Treg in BM and involved lymphatic tissues from patients with B-cell NHL were measured as described earlier. Each open circle represents a single individual assessed in the respective group and numbers on the left of horizontal bars represent the group means.