<p>Gating on CD3+/CD4+ cells (left panel) was followed by subsequent gating according to the CD25 and CD127 expression (right panel). Tregs were defined as the CD4<sup>+</sup>CD25<sup>high</sup>CD127<sup>low/-</sup> -cells (P3). (A) A patient with high Tregs/long T cell telomere length. % Tregs of total PBL: 1.7; % Tregs of CD4+ cells: 7.7; T cell telomere length: 0.65. (B) A patient with low Tregs/short T cell telomere length. % Tregs of total PBL: 0.2; % Tregs of CD4+ cells: 4.8; T cell telomere length: 0.33.</p
<p>(A) CD4<sup>+</sup>CD25<sup>−</sup>, CD4<sup>+</sup>CD25<sup>int</sup> and CD4<sup>+</sup>CD25<su...
<p>Representative contour plots of Tregs (A) (CD25<sup>+</sup>Foxp3<sup>+</sup> of CD4<sup>+</sup> c...
<p>(A) PBMCs were stained with Abs against CD4, CD45RA, and FOXP3, and then analyzed by flow cytomet...
<p><b>A</b>, Expression of CD25, CD127 and FOX-P3 in CD4<sup>+</sup> T cells. Representative dot plo...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>Representative flow cytometry analysis of CD25<sup>+</sup>CD127<sup>low</sup> subpopulations amon...
<p>(<b>A</b>) PBMC were electronically gated on lymphocyte population (left panel) followed by gate ...
<p>A: CD4<sup>+</sup>-T cells (P1) were gated by fluorescence intensity of CD4 versus side light sca...
<p>The frequencies of CD4<sup>+</sup>CD25<sup>high</sup> putative Treg in tumor associated and unaff...
<p>A. Representative examples of gating strategy used for CD25<sup>+</sup>FOXP3<sup>+</sup> staining...
<p>Histograms represent: <b>A</b>, Proportion of CD4<sup>+</sup>CD25<sup>high</sup>CD127<sup>low/−</...
<p>Relative quantification of Treg cells by <i>FOXP3</i> Treg-specific demethylated region (<i>TSDR<...
<p>A. Flow-cytometry gating strategy used to isolate CD25<sup>+</sup>CD127<sup>low</sup> regulatory ...
Representative plots showing non-circulating T follicular helper cells (cTfh) populations as CD3+CD4...
T regulatory cells (Tregs) are a cell subset that can suppress immune responses to maintain homeosta...
<p>(A) CD4<sup>+</sup>CD25<sup>−</sup>, CD4<sup>+</sup>CD25<sup>int</sup> and CD4<sup>+</sup>CD25<su...
<p>Representative contour plots of Tregs (A) (CD25<sup>+</sup>Foxp3<sup>+</sup> of CD4<sup>+</sup> c...
<p>(A) PBMCs were stained with Abs against CD4, CD45RA, and FOXP3, and then analyzed by flow cytomet...
<p><b>A</b>, Expression of CD25, CD127 and FOX-P3 in CD4<sup>+</sup> T cells. Representative dot plo...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>Representative flow cytometry analysis of CD25<sup>+</sup>CD127<sup>low</sup> subpopulations amon...
<p>(<b>A</b>) PBMC were electronically gated on lymphocyte population (left panel) followed by gate ...
<p>A: CD4<sup>+</sup>-T cells (P1) were gated by fluorescence intensity of CD4 versus side light sca...
<p>The frequencies of CD4<sup>+</sup>CD25<sup>high</sup> putative Treg in tumor associated and unaff...
<p>A. Representative examples of gating strategy used for CD25<sup>+</sup>FOXP3<sup>+</sup> staining...
<p>Histograms represent: <b>A</b>, Proportion of CD4<sup>+</sup>CD25<sup>high</sup>CD127<sup>low/−</...
<p>Relative quantification of Treg cells by <i>FOXP3</i> Treg-specific demethylated region (<i>TSDR<...
<p>A. Flow-cytometry gating strategy used to isolate CD25<sup>+</sup>CD127<sup>low</sup> regulatory ...
Representative plots showing non-circulating T follicular helper cells (cTfh) populations as CD3+CD4...
T regulatory cells (Tregs) are a cell subset that can suppress immune responses to maintain homeosta...
<p>(A) CD4<sup>+</sup>CD25<sup>−</sup>, CD4<sup>+</sup>CD25<sup>int</sup> and CD4<sup>+</sup>CD25<su...
<p>Representative contour plots of Tregs (A) (CD25<sup>+</sup>Foxp3<sup>+</sup> of CD4<sup>+</sup> c...
<p>(A) PBMCs were stained with Abs against CD4, CD45RA, and FOXP3, and then analyzed by flow cytomet...