Treg frequencies in tumor and unaffected mucosa.

<p>The frequencies of CD4⁺CD25^high putative Treg in tumor associated and unaffected mucosa from colon cancer patients were investigated using flow cytometry. (A) Paired individual frequencies of CD4⁺CD25^hiputative Tregs among all CD4⁺ T cells from 17 patients, and an example of CD4 and CD25 staining in one individual. (B) Intracellular FOXP3 and surface CD127 staining of CD25^hi cells in the tumor from one individual. (C) Frequencies of CD4⁺CD25^int T cells among all CD4⁺ T cells in unaffected and tumor mucosa. Connected lines represents data from the same individual. (D) Methylation status of the FOXP3 promoter region in FOXP3⁺ and FOXP3⁻ CD4⁺ T cells sorted by flow cytometry from lamina propria cells isolated from unaffected and colon tumor tissue, as determined by Ms-SNUPE. Each symbol represents data from one individual. *** p<0.001, Wilcoxon signed rank test.