Characterization of Treg using flow cytometry in controls and patients.

<p><b>A</b>, Expression of CD25, CD127 and FOX-P3 in CD4⁺ T cells. Representative dot plots for healthy controls (HC), AAV patients in remission phase (AAV-RP) and AAV patients in acute phase (AAV-AP) showing CD25 vs. CD127 and CD25 vs. FOXP3 expression in gated CD4⁺CD3⁺ T cells are shown. The gate use to identify Treg in fresh blood is shown in each CD25 vs. CD127 dot plot. <b>B</b>. Representative histograms showing expression of FOX-P3 (bold line) vs. isotype control (dotted line) and CD39 (inset) in CD4⁺CD25^highCD127 ^low cells as gated in A.