<p>Peripheral lymphocytes were analyzed for CD4-FITC, CD25-PE and Foxp3-APC expressions. The graphs showed that CD4<sup>+</sup> T cells gated by R2 were isolated from lymphocytes and divided into CD4<sup>+</sup>CD25<sup>+</sup>(R3), CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> (R4), CD4<sup>+</sup>CD25<sup>low</sup> (R5) and CD4<sup>+</sup>CD25<sup>high</sup> (R6). Data represented the results of one representative experiment out of seven performed under the same conditions.</p
<p>Single-cell suspensions of nucleated thymocytes from intact NIK KO or WT mice were stained with t...
<p>(<b>A</b>) Peripheral blood mononuclear cells (PBMC) were stained for flow cytometric analysis an...
<p>Splenic lymphocytes were isolated from one NOD mice (total number of mice was 6) and cultured wit...
<p>(A) CD4<sup>+</sup>CD25<sup>−</sup>, CD4<sup>+</sup>CD25<sup>int</sup> and CD4<sup>+</sup>CD25<su...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>Isolated lymphocytes were stained with monoclonal antibodies against CD4, CD25, CD127, FOXP3, CD4...
<p>Representative flow cytometry analysis of CD25<sup>+</sup>CD127<sup>low</sup> subpopulations amon...
<p>(<b>A</b>) PBMC were electronically gated on lymphocyte population (left panel) followed by gate ...
<p>PBMCs extracellular stained with PerCP labeled anti-human CD4-antibody, PE labeled anti CD25-anti...
<p>CD4+ CD25<sup>hi</sup> cells were characterized by flow cytometry. Fig 1A represents a dot plot s...
<p><b>A</b>. CD4<sup>+</sup> T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodi...
<p><b>A</b>) Gating strategy for accurate determination of the proportion of regulatory T cells in P...
<p>A) Absolute numbers from spleens from four-week-old cHET and cKO mice, including total spleen cel...
<p>T cells (n = 5) were stimulated by eight types of DC/tumor (Imm/Mock, OK/Mock, PSK/Mock, OPK/Mock...
It has recently been suggested that a lack of CD127 expression can be used to identify human CD4+ re...
<p>Single-cell suspensions of nucleated thymocytes from intact NIK KO or WT mice were stained with t...
<p>(<b>A</b>) Peripheral blood mononuclear cells (PBMC) were stained for flow cytometric analysis an...
<p>Splenic lymphocytes were isolated from one NOD mice (total number of mice was 6) and cultured wit...
<p>(A) CD4<sup>+</sup>CD25<sup>−</sup>, CD4<sup>+</sup>CD25<sup>int</sup> and CD4<sup>+</sup>CD25<su...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>Isolated lymphocytes were stained with monoclonal antibodies against CD4, CD25, CD127, FOXP3, CD4...
<p>Representative flow cytometry analysis of CD25<sup>+</sup>CD127<sup>low</sup> subpopulations amon...
<p>(<b>A</b>) PBMC were electronically gated on lymphocyte population (left panel) followed by gate ...
<p>PBMCs extracellular stained with PerCP labeled anti-human CD4-antibody, PE labeled anti CD25-anti...
<p>CD4+ CD25<sup>hi</sup> cells were characterized by flow cytometry. Fig 1A represents a dot plot s...
<p><b>A</b>. CD4<sup>+</sup> T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodi...
<p><b>A</b>) Gating strategy for accurate determination of the proportion of regulatory T cells in P...
<p>A) Absolute numbers from spleens from four-week-old cHET and cKO mice, including total spleen cel...
<p>T cells (n = 5) were stimulated by eight types of DC/tumor (Imm/Mock, OK/Mock, PSK/Mock, OPK/Mock...
It has recently been suggested that a lack of CD127 expression can be used to identify human CD4+ re...
<p>Single-cell suspensions of nucleated thymocytes from intact NIK KO or WT mice were stained with t...
<p>(<b>A</b>) Peripheral blood mononuclear cells (PBMC) were stained for flow cytometric analysis an...
<p>Splenic lymphocytes were isolated from one NOD mice (total number of mice was 6) and cultured wit...