The gates for CD25and CD4CD25T cells were set based on the expression level of CD25 on CD4T cells, where the pattern of CD25 expression is more distinct. Accordingly, CD4CD25T cells were defined as those expressing CD25 at a level higher than the bulk CD4CD25population. For D-K, individual results are represented in symbols, and the line represents the mean. (D) % CD4CD25T cells; (E) % CD4CD25T cells; (F) % CD4CD25CTLA-4T cells; (G) % of CD4CD25CTLA-4T cells; (H) % CD4CD25CD127T cells; (I) % CD4CD25CD127T cells; (J) % CD4CD25GITRT cells; and (K) % CD4CD25GITRT cells.<p><b>Copyright information:</b></p><p>Taken from "The frequency of CD127expressing CD4CD25T regulatory cells is inversely correlated with human T lymphotrophic virus type-1 (HT...
<p>All lymphocytes (gate O) were gated by forward scatter/side scatter (FS/SS), differentiating T ce...
<p>PBMCs were cultured overnight with or without anti-CD3 stimulation. Change in CD25 or CD86 expre...
<p><b>A.</b> Representative dot plot showing gating strategy for immunophenotyping of the three subp...
<p>Lymphocytes were gated according to forward and side scatter. CD3 positive lymphocytes were selec...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>CD4+ CD25<sup>hi</sup> cells were characterized by flow cytometry. Fig 1A represents a dot plot s...
(A) For panel 6, the strategies of the first to fourth gates were the same as panel 4 (Fig 4). After...
<p>Different strategies for gating regulatory T cells in peripheral blood and lymph node respectivel...
For panel 8, the strategies of the first to fifth gates were the same as in panels 5 and 6 (Figs 4B ...
<p>(A) Plots showing the sorting strategy for CD4<sup>+</sup>CD25<sup>hi</sup>, CD4<sup>+</sup>CD25<...
<p>These plots represent the gating strategy used for flowcytometry analysis of blood samples; lymph...
<p>A) Forward Scatter vs Side Scatter B) CD3 versus CD4 within the lymphocyte gate C) FOXP3 versus C...
<p>A) Representative flow cytometry dot-plot showing lymphocytes gated physically. B) Representative...
(A) For panel 4, the first and second gates were the same as panel 2 (Fig 3), the third gate was on ...
<p>All flow cytometry plots are from a single subject of the RESTORE study. Lymphocytes were identif...
<p>All lymphocytes (gate O) were gated by forward scatter/side scatter (FS/SS), differentiating T ce...
<p>PBMCs were cultured overnight with or without anti-CD3 stimulation. Change in CD25 or CD86 expre...
<p><b>A.</b> Representative dot plot showing gating strategy for immunophenotyping of the three subp...
<p>Lymphocytes were gated according to forward and side scatter. CD3 positive lymphocytes were selec...
<p>(A) Dot plots were gated on CD4<sup>+</sup> T cells (based on forward and side scatter and CD4 st...
<p>CD4+ CD25<sup>hi</sup> cells were characterized by flow cytometry. Fig 1A represents a dot plot s...
(A) For panel 6, the strategies of the first to fourth gates were the same as panel 4 (Fig 4). After...
<p>Different strategies for gating regulatory T cells in peripheral blood and lymph node respectivel...
For panel 8, the strategies of the first to fifth gates were the same as in panels 5 and 6 (Figs 4B ...
<p>(A) Plots showing the sorting strategy for CD4<sup>+</sup>CD25<sup>hi</sup>, CD4<sup>+</sup>CD25<...
<p>These plots represent the gating strategy used for flowcytometry analysis of blood samples; lymph...
<p>A) Forward Scatter vs Side Scatter B) CD3 versus CD4 within the lymphocyte gate C) FOXP3 versus C...
<p>A) Representative flow cytometry dot-plot showing lymphocytes gated physically. B) Representative...
(A) For panel 4, the first and second gates were the same as panel 2 (Fig 3), the third gate was on ...
<p>All flow cytometry plots are from a single subject of the RESTORE study. Lymphocytes were identif...
<p>All lymphocytes (gate O) were gated by forward scatter/side scatter (FS/SS), differentiating T ce...
<p>PBMCs were cultured overnight with or without anti-CD3 stimulation. Change in CD25 or CD86 expre...
<p><b>A.</b> Representative dot plot showing gating strategy for immunophenotyping of the three subp...