Peritrophin mRNA expression profiles.

<p>Expression of <i>PpPer1</i>, <i>PpPer2</i>, and <i>PpPer3</i> mRNAs was assessed by RT-PCR (23–25 cycles) in <i>P. papatasi</i> midguts dissected from adult females at different time points before and after blood feeding (0–144 hours PBM) (<b>A</b>); in pools of tissues other than midgut (<b>B</b>); and in eggs (pool of 20 eggs) or whole body of larvae and pupae (<b>C</b>). β-Tub was used as the housekeeping control gene. The size of the cDNA fragments amplified were 122 bp (<i>PpPer1</i>), 168 bp (<i>PpPer2</i>), 121 bp (<i>PpPer3</i>), and 148 bp (β-Tub). CC: Carcass. HG: Hindgut. FB: Fat Body. HS: Head along with salivary glands. OV: Ovaries. MT: Malpighian Tubules. E: Eggs. L1: Larval stage 1. L2: Larval stage 2. L3: Larval stage 3. L4: Larval stage 4. P: Pupa. (-): Negative control.