Expression of Pgp mRNAs during <i>H. contortus</i> life cycle.

<p>Real-time RT-PCR experiments were performed in triplicate for each developmental stage: Eggs (E) and third stage larvae (L3) corresponding to the free-living stages as well as in fourth stage larvae (L4) and adult males (A) corresponding to parasitic stages. The mRNA expression levels observed in the eggs was normalized to 1. The mRNA fold changes were calculated using three distinct reference genes (<i>gapdh, actin</i> and <i>β-tubulin</i>). For each sample, real-time RT-PCR were performed in triplicate and repeated twice using two independent cDNAs templates. * Significant difference from control values after Bonferroni's Multiple Comparison Test (* P<0.05; ** P<0.01; *** P<0.001).