Expression profiles of members of <i>CPAP</i> gene families (<i>CPAP1</i> and <i>CPAP3</i>) as determined by RT-PCR.

<p>Approximately 500 eggs (14.9 mg) were collected and used for RNA isolation. Pools of four larvae, (young and old separately) pharate pupae, pupae from day-0 to day-5, young adults (∼1–2 hour old) or mature adults (>2 weeks-old) were used for preparation of total RNA at these stages. cDNAs synthesized from total RNA using the oligo-(dT) primer and reverse transcriptase were used as templates for RT-PCR (28 cycles).The initial denaturation was at 94°C for 10 minutes, annealing at (56°C–60°C) for 1 minute and the final extension was at 72°C for 10 minutes, using gene-specific primers. RT-PCR products for the <i>T. castaneum</i> ribosomal protein S6 gene (<i>RpS6</i>) from the same cDNA templates served as an internal control for loading (24 cycles). (A) Expression profiles of <i>CPAP1</i> genes at different stages of development: E, eggs; YL, penultimate instar or younger larvae; ML, mature (last instar) larvae; PP, pharate pupae; P, pupae; YA, young adult; 3 hours after eclosion; MA, mature adult; 10 days after eclosion. (B) Expression profiles of <i>CPAP1</i> genes at different stages of pupal development. P_[0–5], pupae 0-day old to pupae 5-days old were collected at 1-day intervals. (C) Expression profiles of <i>CPAP3</i> genes at different stages of development: E, eggs; YL, penultimate instar or younger larvae; ML, mature (last instar) larvae; PP, pharate pupae; P, pupae; YA, young adult; 3 hours after eclosion; MA, mature adult; 10 days after eclosion. (D) Expression profiles of <i>CPAP3</i> genes at different stages of pupal development. P_[0–5], pupae 0-day-old to pupae 5-days-old were collected at 1-day intervals.