Expression of PpPer1 and PpPer3 in <i>P. papatasi</i> midgut lysates.

<p>Expression of native PpPer1 and PpPer3 in <i>P. papatasi</i> midgut lysates was assessed using one midgut equivalent (from pools of five guts for each time point) of non blood fed and blood fed <i>P. papatasi</i> per lane. Lanes: M, size marker; 1, non blood fed midgut; 2, blood fed midgut dissected 24 h PBM; 3, blood fed midgut dissected 48 h PBM; 4, blood fed midgut dissected 72 h PBM; 5, blood fed midgut dissected 96 h PBM. Proteins were transferred to nitrocellulose and blots were incubated with anti-PpPer1 (A) and with anti-PpPer3 (B) specific antisera. Arrows point the native proteins PpPer1 (A) and PpPer3 (B).