<p>qRT-PCR assays depicting differences in PpPer1 (<b>A</b>), PpPer2 (<b>B</b>), and PpPer3 (<b>C</b>) mRNA levels between non-infected and <i>Le. major</i> infected midguts dissected at 24 h, 48 h, and 72 h PBM. Each dot (symbol) represents the mRNA expression levels in a single midgut whereas horizontal bars indicate mean expression levels. The cDNA encoding the S3 protein of the 40S ribosomal subunit was used as the housekeeping control gene. The mean expression of non-infected midguts was used as a standard (100%) for comparisons to the percentage of mRNA expression of <i>Le. major</i> infected midguts for each time point. NI: Non-infected. INF: <i>Le. major</i> infected. NS: Not significant. *: Statistically significant, p<0.05.</p
<p>(A). Change of the LA0543-, LA2250- or LB361-mRNA level in <i>L. interrogans</i> strain Lai durin...
<p>Midguts were dissected from non-blood fed females and incubated for 3 h in saline (Sal), amino ac...
<p>The bar chart represents the quantification of the PCR results that corresponds to the mRNA level...
<p>Expression of <i>PpPer1</i>, <i>PpPer2</i>, and <i>PpPer3</i> mRNAs was assessed by RT-PCR (23–25...
<p>Knockdown of PpPer1 leads to greater <i>Le. major</i> load in the midgut of <i>P. papatasi</i>. A...
<p>Quantitative (Q) RT-PCR analysis of <i>REG3γ</i> and <i>IL8</i> mRNA levels in human gastric epit...
<p>Indicated is the fold change in expression of selected genes between persistent and pathogenic in...
<p>Expression of native PpPer1 and PpPer3 in <i>P. papatasi</i> midgut lysates was assessed using on...
<p>(A) Relative transcript levels of <i>nub-RB</i> (yellow) and <i>nub-RD</i> (magenta) in extracts ...
<p>The infection index (A, B and C), the <i>5</i>.<i>1 aap3</i> copy number (D, E and F) and the <i>...
<p>A. Clustering of differential expression of genes with the use of control as a standard. Red deno...
<p>(A) Light micrograph of midguts dissected from an intact and decapitated female at 16 h post bloo...
<p>TGFβ1 mRNA was measured by RT-PCR. mRNA levels increased in Groups 2 and 4 compared to Group 1 bu...
<p>Genes were clustered based on main functions relative to milk fat synthesis. Ad(control)-infected...
<p>The bar charts represent the quantification of the PCR results that correspond to the mRNA levels...
<p>(A). Change of the LA0543-, LA2250- or LB361-mRNA level in <i>L. interrogans</i> strain Lai durin...
<p>Midguts were dissected from non-blood fed females and incubated for 3 h in saline (Sal), amino ac...
<p>The bar chart represents the quantification of the PCR results that corresponds to the mRNA level...
<p>Expression of <i>PpPer1</i>, <i>PpPer2</i>, and <i>PpPer3</i> mRNAs was assessed by RT-PCR (23–25...
<p>Knockdown of PpPer1 leads to greater <i>Le. major</i> load in the midgut of <i>P. papatasi</i>. A...
<p>Quantitative (Q) RT-PCR analysis of <i>REG3γ</i> and <i>IL8</i> mRNA levels in human gastric epit...
<p>Indicated is the fold change in expression of selected genes between persistent and pathogenic in...
<p>Expression of native PpPer1 and PpPer3 in <i>P. papatasi</i> midgut lysates was assessed using on...
<p>(A) Relative transcript levels of <i>nub-RB</i> (yellow) and <i>nub-RD</i> (magenta) in extracts ...
<p>The infection index (A, B and C), the <i>5</i>.<i>1 aap3</i> copy number (D, E and F) and the <i>...
<p>A. Clustering of differential expression of genes with the use of control as a standard. Red deno...
<p>(A) Light micrograph of midguts dissected from an intact and decapitated female at 16 h post bloo...
<p>TGFβ1 mRNA was measured by RT-PCR. mRNA levels increased in Groups 2 and 4 compared to Group 1 bu...
<p>Genes were clustered based on main functions relative to milk fat synthesis. Ad(control)-infected...
<p>The bar charts represent the quantification of the PCR results that correspond to the mRNA levels...
<p>(A). Change of the LA0543-, LA2250- or LB361-mRNA level in <i>L. interrogans</i> strain Lai durin...
<p>Midguts were dissected from non-blood fed females and incubated for 3 h in saline (Sal), amino ac...
<p>The bar chart represents the quantification of the PCR results that corresponds to the mRNA level...