Amino acids enhance ILP3 stimulation of <i>AaSPVI</i> expression in midguts in vitro.

<p>Midguts were dissected from non-blood fed females and incubated for 3 h in saline (Sal), amino acid supplemented saline (AA), saline plus 20 pmol ILP3 (sal + ILP3), saline plus ILP3 and 100 pmol rapamycin (sal+ILP3+Rap), amino acid saline plus ILP3 (AA+ILP3) or amino acid saline plus ILP3 and rapamycin (AA+ILP3+Rap). rqRT-PCR analysis was then performed to determine relative transcript abundance of <i>AaSPVI</i>. Transcript levels were standardized to 1 for the saline-treated sample (negative control) while transcript abundance for the other treatments is expressed relative to the negative control. Each treatment was replicated three times using midguts from four females.