Relative expression of <i>pbphlp-3</i> in different parasite stages.

Relative transcript abundance of pbphlp-3 and the control genes thioredoxin-dependent peroxidase-1 (tpx-1) and 1-cys peroxiredoxin (1-cys prx) in A. asexual P. berghei parasites from mouse blood B. mosquito stage parasites developing either in the mosquito midgut or under culture condition. Indicated fold changes specify the difference to pbphlp-3 expression. ** signify statistical significance with a p-value C. Temporal transcription profile of pbphlp-3 in developing mosquito-stage parasites during the first 24 hours in mosquito midguts or under culture conditions, respectively. For the investigation of mosquito stage parasites female mosquitoes were allowed to feed on infected mice and blood fed midguts were subsequently dissected at 3, 6, 12, and 24 hours post blood meal. For ookinete cultures, samples were taken at the same time points as the midgut dissections. Error bars represent standard deviation of three independent experiments.