The CAF05 adjuvant enhances the cytotoxicity of CD8 T cells.

<p>Mice were immunized three times i.p. with 20 µg Gag p24 protein alone (‘no adjuvant’) or adjuvanted in either CAF01 or CAF05. Three weeks later <i>in vivo</i> cytotoxicity was determined 1 day after adoptive transfer of AMQMLKETI-pulsed (CFSE^hi) or unpulsed (CFSE^lo) CFSE-stained target cells from naïve mice. (A) The specific lysis relative to the naïve control group. Bars represent the mean specific lysis of individual mice within each group (n = 3/4+/− SEM). Significant differences as determined by one-way ANOVA followed by Tukey’s post test have been indicated: *<i>p<</i>0.05, ***<i>p<</i>0.001. (B) Gating strategy for one representative mouse. (C) Representative histograms of CFSE^lo and CFSE^hi populations for all three immunization groups.