One microgram of adjuvant/formulated-antigens generates a higher specific cellular immune response than five micrograms.

<p>Groups of BALB/c mice were immunized 3 times i.m. (separated by 4 weeks each) with 2 different doses of adjuvant/formulated-antigens: 1 μg and 5 μg of each individual component (VPA). Non-adjuvanted formulated antigens (VP; 1 μg of each antigen) or PBS were injected in animals from the 2 control groups. Four weeks after the last immunization, animals were euthanized and their spleens and sera collected. (i) Specific CD4⁺ and CD8⁺ T cell proliferation was assessed by Flow Cytometry four days after CFSE-stained splenocytes-culture in the presence of LJL143, LeishF3 or KMP11 (10 μg/ml). (ii) Serum antigen specific IgG titers for LJL143 were determined by ELISA. Each dot represents one animal. Average and SD of the values within each group are shown. Statistical differences are properly identified (Unpaired t-test: * p≤0.05, ** p≤0.01, *** p≤0.001 and **** p≤0.0001).