Serum antibody responses in BALB/c mice immunized with rEm-TSP3 either subcutaneously or intranasally.

<p>BALB/c mice were immunized with recombinant Em-TSP3 at a dose of 50 µg/mouse plus adjuvant (CpG for the intranasal group or Freund's complete adjuvant (CFA) or Freund's incomplete adjuvant (IFA) for the subcutaneous group). After the third immunization, blood was collected and sera IgG (A), IgG1 (B), IgG2α (C), IgM (D) and IgA (E) were assayed by ELISA. Results represent the mean absorbance measured at 450 nm for each group. Significant differences between the vaccinated groups and the PBS control group are denoted by an asterisk over the bar. Significant differences between any of the groups are denoted by an asterisk over the line connecting them. n = 8 per group; s.c. = subcutaneous; i.n. = intranasal; adj. = adjuvant IFA/CFA. *<i>p</i><0.05 (significant); **<i>p</i><0.01 (very significant); ***<i>p</i><0.001 (extremely significant).