Selective T cell response of vaccinated mice.

<p>^a Number of IFNγ secreting cells/10⁶ splenocytes (splenocytes were removed 14 days following the last immunization (see Materials and Methods)). Data represents mean and SD from 3 individual animals (per group) derived from 3 independent experiments.</p><p>^b 10⁷ CFU/ml of formalin-inactivated LVS (see Materials and Methods).</p><p>^c 10 µg of anti-CD4 antibodies were added to 10⁶ splenocytes 1 hour before stimulation with formalin inactivated LVS.</p><p>^d 10 µg of anti-CD8 antibodies were added to 10⁶ splenocytes 1 hour before stimulation with formalin inactivated LVS.</p><p>^e No-Ag, samples were tested without any added antigen.</p><p>^f mice were immunized by gene gun. (See Materials and Methods).