Immunological responses induced in Balb/c mice by vaccine formulations using SE, Alum or TLR ligands.

<p>Groups of 10 Balb/c mice were immunized with 2 doses of 2.5 µg CSP, 2 months apart in the indicated adjuvant. Two weeks post second immunization, the mice were bled and the serum was analyzed. <i>A,</i> ELISA end point titers of Balb/c mice, measured against CS/D (left) or NANP repeat peptide (right). <i>B,</i> IgG1 (left) and IgG2a (right) subclasses measured by Luminex and expressed as mean fluorescence intensities (MFI) at 1∶500 serum dilution. <i>C,</i> Percentage of total cytokine⁺CD44⁺CD4⁺ T lymphocytes, extracted from vaccinated Balb/c mice and stimulated with CS/D, stained for surface expression of CD3, CD4 and CD44 and intra-cellular expression of IL-2 (left) or IFN-γ (right). Lines are mean with SEM. (*) indicates significant P values for ANOVA followed by Tukey’s multiple comparison test.