Immunological responses induced in Balb/c mice by CS/D covalently conjugated to 3M-051.

<p>Groups on 10 Balb/c mice were immunized two doses of 2.5 µg CSP, 2 months apart in the indicated adjuvant. One month post second immunization sera were collected and analyzed. <i>A,</i> ELISA end point titers of mice, measured against CS/D (left) or NANP repeat peptide (right). <i>B,</i> IgG1 (left) and IgG2a (right) subclasses expressed as Luminex mean fluorescence intensities (MFI) at 1∶500 serum dilution. <i>C</i>, Percentage of total Cytokine⁺CD44⁺CD4⁺ T lymphocytes, extracted from vaccinated Balb/c mice and stimulated with CS/D, stained for intracellular IL-2 (left) or IFN-γ (middle) or CD8⁺ cells stimulated with the K^d-restricted peptide and stained for IFN-γ (right). Lines are mean with SEM.