Prime boost immunogenicity studies.

<p>BALB/c mice (n = 20 mice/group) were immunized with DNA or adenovectors as indicated. (A) Schematic of the experiment. (B) <i>Py</i>CSP-specific T cell responses were assessed from a subset of the mice (n = 6) by IFNγ ELISpot two weeks after immunization. Targets were MHC-matched A20.2J cells pulsed with synthetic peptides representing the immunodominant CD8⁺ T cell epitope (<i>Py</i>CSP280–288), CD4⁺ T cell epitope with nested CD8+ T cell epitope (<i>Py</i>CSP280–296), the immunodominant CD4⁺ T cell epitope (<i>Py</i>CSP57–70), the subdominant CD8⁺ T cell epitope (<i>Py</i>CSP58–67), and CD4⁺ T cell epitope with nested CD8+ T cell epitope (<i>Py</i>CSP58–79) from <i>Py</i>CSP. Negative control values were subtracted from reported data. Error bars indicate the standard deviation of the mean from quadruplicate samples. (C) ELISA against <i>Py</i>CSP repeat peptide capture antigen. Error bars indicate the standard error of the mean.