Cellular immune responses are induced to a similar level after immunization with IFA and CAF01.

<p>IFN-γ ELISPOT responses against (A) HLA-A2-restricted CD8 T cell epitope Vif₁₀₁ and (B) PADRE Th epitope of splenocytes from HLA-A*0201 transgenic HHD mice after 10 days of s.c. immunization with Vif₁₀₁ and PADRE in either IFA (grey bars, n = 5) or CAF01 adjuvants (black bars, n = 5) and 5 days <i>in vitro</i> prestimulation with individual epitopes. The background of an unimmunized mouse is substracted. Specific ⁵¹Cr-release from target cells preloaded with Vif₁₀₁ after incubation with effector splenocytes from Vif₁₀₁ and PADRE immunized mice adjuvanted with (C) IFA (grey, n = 5) or (D) CAF01 (black, n = 5). The percentage of specific lysis was calculated as 100×(experimental release-spontaneous release)/(total release-spontaneous release). Background lysis from an unimmunized mouse is shown (open circles). Significant positive levels are considered for >10% lysis at a 50∶1 ratio of effector:target (E:T) cells. One representative experiment out of three. SFU, spot forming units.