Targeting of Ag85B-ESAT-6 and CAF01 in neonatal and adult DC of the draining LN.

<p>Twenty-four hours after immunization, (A) the percentage of CAF01-NBD⁺ CD11c⁺ cells compared to all cells bearing the adjuvant in the draining LN was determined. In a representative dot plot of an adult mouse, the percent of cells in quadrants is shown. (B) The percent of Ag85-ESAT-6⁺ CAF01⁺ DC amongst all CAF01⁺ DC was determined. In a representative histogram from an adult mouse, the presence of Ag85-ESAT-6-FITC in CAF01⁺ DC (thick line) was calculated using total DC as the control histogram (dotted line). The percent (C) and number (D) of Ag85-ESAT-6⁺ CAF01⁺ DC was calculated. The data are expressed as mean and SD of groups of at least 4 individual mice, and is representative of 4 independent experiments. *, p<0.05 signifies differences between neonatal and adult mice.