SU5416 treatment reduces immune responses.

<p>A) Mice were immunized unilaterally as described in Fig. 1. Mice were treated with SU5416 (25 mg/kg/day) or vehicle control starting on the day of immunization. After 3 days, mice were pulsed with BrdU for 1 hour, control and draining PLN were harvested and labeled with fluorochrome-conjugated antibodies to detect CD45 and BrdU, and analyzed by flow cytometry. Values represent the mean ± SEM percentage or number of CD45⁺ BrdU⁺ cells in each tissue from 4–5 mice per group. B) Mice were immunized in all limbs on days 0 and 28 with DNP-KLH-Alum and treated with SU5416 (50 mg/kg, twice per week) or vehicle control. Serum was collected 7, 14, 21 and 35 days following initial immunization and analyzed by isotype-specific ELISA for the presence of DNP-specific antibodies. Values represent the mean ± SEM levels of DNP-specific IgM and IgG1 antibodies from 5 mice per group. *Differences in the mean values between SU5416 and vehicle control treatments were significant; p<0.05.