<p>A) Evaluation of the Class II high populations of neonatal (7 days old) vs. adult mice shows differences in composition and phenotype of DC populations. B) Costimulatory molecule (CD86, CD80 and CD70) expression was measured by flow cytometry on CD103+ and CD11b+ DCs three days post-infection at the indicated age and the mean fluorescence intensity (MFI) is displayed. Data are representative of three experiments using two to three samples of lymph nodes pooled from 3–7 mice. Error bars indicate the SEM, and all data was analyzed using one-way ANOVA with Tukey's multiple comparisons test (* p≤0.05, ** p≤0.01, *** p≤0.001, **** p≤0.0001).</p
<p>Mouse DC cultures were sorted into TM<sup>+</sup> and TM<sup>−</sup> DCs before analysis of MHC I...
<p>(A and B) Young and old mice were intravenously injected with 100 μg polyU/DO. Eighteen hours lat...
<p>(A) Filled histograms show expression of CD86 and MHC-II by gastric macrophages (Gr1<sup>−</sup>C...
<p>CD103+ and CD11b+ DC populations in the MLN three days post-RSV infection were measured in mice o...
<p>Flow cytometric analysis of cells isolated from mesenteric lymph nodes (MLN), pancreatic lymph no...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>RIG-I<sup>+/+</sup> and RIG-I<sup>-/-</sup> mice were infected with 50 PFU of PR8 and CD86 expres...
Mice were infected A) Frequency and number of cDCs (CD11c+ PDCA-1−) and pDCs (CD11clow PDCA-1+) iden...
<p>(<b>A</b>) Sections of the small intestine of mice at different ages: 7 days old, 13 days old, 13...
<p>(A-C) Adult (6 week old) and infant (7d old) mice were inoculated with strain P1121 for the indic...
<p>Lung lymphocytes from mice sacrificed at different stages of infection (Early: week 6; late: week...
<p>Expression of the activation markers (A) B7.2 and MHCII was quantified by mean fluorescent intens...
<p>(A) Representive images of CD80 mean fluorescence intensity (MFI) in CD11b<sup><i>+</i></sup> cel...
<p>Mouse littermates were infected at 6, 12 or 16 months of age with MCMV (red symbols) or VACV-IE1 ...
<p>Analysis of lung DC subsets in (A–C) 6–8 week-old and (D–E) 6-day-old mice. (A, D) Gated cells in...
<p>Mouse DC cultures were sorted into TM<sup>+</sup> and TM<sup>−</sup> DCs before analysis of MHC I...
<p>(A and B) Young and old mice were intravenously injected with 100 μg polyU/DO. Eighteen hours lat...
<p>(A) Filled histograms show expression of CD86 and MHC-II by gastric macrophages (Gr1<sup>−</sup>C...
<p>CD103+ and CD11b+ DC populations in the MLN three days post-RSV infection were measured in mice o...
<p>Flow cytometric analysis of cells isolated from mesenteric lymph nodes (MLN), pancreatic lymph no...
<p>Spleen (SPL) (<b>A</b>) and skin draining lymph nodes (SDLN) (<b>B</b>) of 8 weeks old WT and DKO...
<p>RIG-I<sup>+/+</sup> and RIG-I<sup>-/-</sup> mice were infected with 50 PFU of PR8 and CD86 expres...
Mice were infected A) Frequency and number of cDCs (CD11c+ PDCA-1−) and pDCs (CD11clow PDCA-1+) iden...
<p>(<b>A</b>) Sections of the small intestine of mice at different ages: 7 days old, 13 days old, 13...
<p>(A-C) Adult (6 week old) and infant (7d old) mice were inoculated with strain P1121 for the indic...
<p>Lung lymphocytes from mice sacrificed at different stages of infection (Early: week 6; late: week...
<p>Expression of the activation markers (A) B7.2 and MHCII was quantified by mean fluorescent intens...
<p>(A) Representive images of CD80 mean fluorescence intensity (MFI) in CD11b<sup><i>+</i></sup> cel...
<p>Mouse littermates were infected at 6, 12 or 16 months of age with MCMV (red symbols) or VACV-IE1 ...
<p>Analysis of lung DC subsets in (A–C) 6–8 week-old and (D–E) 6-day-old mice. (A, D) Gated cells in...
<p>Mouse DC cultures were sorted into TM<sup>+</sup> and TM<sup>−</sup> DCs before analysis of MHC I...
<p>(A and B) Young and old mice were intravenously injected with 100 μg polyU/DO. Eighteen hours lat...
<p>(A) Filled histograms show expression of CD86 and MHC-II by gastric macrophages (Gr1<sup>−</sup>C...