Anti-CD8 mAbs have direct effects on target recognition and indirect effects on cytotoxic function.

(A-B) IncuCyte cytotoxicity assays presented as the ratio of target cell death/total target cells over time. Activated OT1 T cells were added to previously plated B16.OVA.RFP tumor target cells. (A) in vitro SIINFEKL activated OT1 T cells were plated at an effector to target ratio of 10:1. Anti-CD8 mAbs were then added at multiple concentrations with at least 4 technical replicates per condition. Data is representative of 3 independent experiments. (B) OT1 T cells were adoptively transferred into naïve mice that were anti-CD8 treated the next day. The following day the mice were immunized and the OT1 T cells FACS isolated from pooled lymph nodes and spleens 7 days later. OT1 T cells were then plated at an effector to target ratio of 1:1 with at least 3 technical replicates per treatment group. Data is representative of 2 independent experiments.