Relative expression of selected genes in ADKD and ADOX WT and OB mice.

<p>10-week-old B6 male mice were chow-fed, intravenously injected with ADKD and ADOX vectors and sacrificed at 3 days p.t, following a 12-hr fast. Expression levels were determined by qPCR normalized to expression levels of the 36B4 housekeeping gene. Fold changes are relative to the <i>GFP</i> control in the same state as the <i>Ildr2</i> (either fasted or refed compared to fasted or refed). * indicates p<0.05; ** indicates p<0.01 (2 tailed t-test). (A) Expression in wild-type mice, 3 days p.t. with adenovirus knockdown vector expressing RNAi for <i>lacZ</i> or <i>Ildr2</i>; (B) Expression in wild-type mice, 10 days p.t. with adenovirus knockdown vector expressing RNAi for <i>lacZ</i> or <i>Ildr2</i>; (C) Expression in wild-type mice, 10 days p.t. with adenovirus vector over-expressing <i>GFP</i> or <i>Ildr2</i>; (D) Expression in wild-type mice, 3 days p.t. with adenovirus knockdown vector expressing RNAi for <i>lacZ</i> or <i>Ildr2:</i> (E) Expression in <i>ob/ob</i> mice, 10 days p.t. with adenovirus knockdown vector expressing RNAi for <i>lacZ</i> or <i>Ildr2</i>; (F) Expression in <i>ob/ob</i> mice, 10 days p.t. with adenovirus vector over-expressing <i>GFP</i> or <i>Ildr2</i>. Changes in transcriptional profiles appear to be secondary to changes in lipid content.