Liver morphology and histology in ADKD and ADOX WT and OB mice.

<p>Chow-fed, 10-week-old B6 males were sacrificed after 24-hr fast (Fasted) or following a 24-hr fast and 12-hr refeeding (Refed). Liver morphology is shown in the upper panels and hematoxylin and eosin staining of representative sections is shown in the lower panels at 200X magnification (scale is 100 µm). Asterisk (*) identifies large droplet, macrovesicular lipid vacuoles, particularly evident in Ob sections; large open arrows (M-D) denote intra-hepatocellular Mallory-Denk-like eosinophilic material; open yellow arrows (mF) denote small droplet, microvesicular fat within hepatocytes; short double black arrows (iMO) indicate mononuclear inflammatory cells, consistent with lymphocytes; large blue arrows (ap) indicate apoptotic hepatocytes; (glyc) identifies a “clear”-appearing hepatocyte with increased glycogen content (e.g., ADOX WT 10d Refed); Portal Tract (or PT) is above the hatched line in ADKD WT 10d Fasted); (CV) is Central Vein; (PV) is Portal Vein; (BD) is Bile Duct. (A) Wild-type mice, 3 days p.t. with adenovirus knockdown vectors expressing RNAi for <i>lacZ</i> or <i>Ildr2</i> (B) Wild-type mice, 10 days p.t. with adenovirus knockdown vectors expressing RNAi for <i>lacZ</i> or <i>Ildr2</i> (C) <i>ob/ob</i> mice, 10 days p.t. with adenovirus knockdown vectors expressing RNAi for <i>lacZ</i> or <i>Ildr2</i> (D) Wild-type mice, 3 days p.t. with adenovirus vector over-expressing <i>GFP</i> or <i>Ildr2</i>; there is no significant steatosis or inflammation (E) Wild-type mice, 10 days p.t. with adenovirus vector over-expressing <i>GFP</i> or <i>Ildr2</i> (F) <i>ob/ob</i> mice, 10 days p.t. with adenovirus vector over-expressing <i>GFP</i> or <i>Ildr2</i>. As described in the text, increased apoptosis without inflammation is consistent with a primary role for ILDR2 in ER stress responses.