The DKO mice have increased IRS1 levels and insulin signaling.

<p>Three-month old mice were fasted overnight, injected (ip) either with insulin or with saline (fasted control), and mice were euthanized for tissue harvest. Cell lysates or total RNAs were prepared from white fat and muscle. A. Total RNAs isolated from white fat and muscle of fasted mice was used for Northern blot analyses. B. Tissue lysates of white fat and muscle of fasted and insulin treated mice were subjected to immunoblot analyses. C. DKO mice exhibited higher blood adiponectin levels. * p<0.05, ** p<0.005, and *** p<0.0005. D. Immunoblot analyses with AMPK and phospho-AMPK antibodies were performed.