Liver, fat and muscle of Tsc1−/− mice show blunted response to insulin.

<p>Following an eight-hour fast, mice were injected with 0.5 U/kg of insulin or saline (control) for 10 minutes before sacrifice. A) Liver and B) white adipose tissue (WAT) and skeletal muscle tissue lysates were analyzed for the expression of indicated proteins. C. Akt response to insulin. The relative band intensities of p-Akt(Ser473) were normalized to total Akt from (A) and (B), and then the ratios between the insulin- and saline-treated animals were calculated from individual tissues tested.