Protection from Influenza A Virus challenge.

<p>Balb/c mice were immunized according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072217#pone-0072217-t001" target="_blank">Table 1</a>. Five weeks after the second immunization, the mice were challenged with 250 PFU IAV (PR/8/34). The weight loss of each animal was monitored daily and the means and SEM (n = 5–6) are indicated for each group up to day 6 post challenge (<b>A</b>). At day 6, viral loads in lung homogenates (vRNA copies/ml) were measured by qRT-PCR. Each animal is represented by one dot and the means of each group are marked by the line. The dotted line indicates the detection limit (428 copies/ml) (<b>B</b>). (p<0.05): <b>*</b> vs. naïve; <b>^#</b> vs. (F)^hi (1 way-ANOVA, Tukey post-test).