EP67 displays protection against influenza-induced morbidity.

<p>Four groups of mice were infected with a non-lethal dose of influenza (0.33×MLD₅₀) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0040303#s2" target="_blank">Materials and Methods</a>. Three groups were treated one time with EP67, either the day before infection (EP67 day −1), at the time of infection (EP67 day 0), or the day after infection (EP67 day 1). Mice were weighed daily. A. Weight change following influenza infection. Group average weight change is shown as mean ± SEM. Day 8 weight loss results are statistically significant (using a two-tailed Student’s t-test) as reported in the text. One experiment is shown; experiment was repeated twice. B. Viral load in lungs was measured using quantitative RT-PCR of the influenza matrix gene. Lung RNA was isolated three days after infection. Results are shown as matrix gene copies per 100 ng RNA. (^∧p<0. 0005, two-tailed Student’s t-test). C. ELISA analysis of anti-influenza antibodies. Individual serum samples were analyzed for total IgG, IgG1, IgG2b, and IgG2c distribution. Results are displayed as mean ± SEM. The total IgG response shown is from the 1∶10,000 dilution of serum. The subclass responses shown are from the 1∶1000 serum dilution. *p<0.05, two-tailed Student’s t-test.