Protective efficacy of AdV vector-based vaccines against challenge with influenza viruses from H5, H7, and H9 subtypes.

<p>Mouse groups were immunized with 1203HA, 05HA, H5b, H7HA, H9HA, H7H9b, H5b+H7H9b or ΔE1E3 twice at four-week intervals. Four weeks post-booster inoculation, the mice (5 animals/group) were challenged with one of the following viruses: Egypt/08 (a), TK/VA (b), or G1/99 (c). Three days after challenge, the animals were euthanized, and the lung virus titers were determined as described under Materials and Methods. The data represent the mean virus titers ± standard deviation (SD). The detection limit of the lung viral titer was 0.5 Log₁₀ TCID₅₀/ml. 1203HA, HAd-1203HA; 05HA, HAd-05HA; H5b, HAd-1203HA-05HA; H7HA, HAd-H7HA; H9HA, HAd-H9HA; H7H9b, HAd-H7HA-H9HA; H5b+H7H9b; HAd-1203HA-05HA+HAd-H7HA-H9HA; ΔE1E3, HAd-ΔE1E3; Egypt/08, A/Egypt/3300-NAMRU3/2008 (H5N1)-PR8-IDCDC-RG13; TK/VA, A/turkey/Virginia/2002 (H7N2)-PR8-IBCDC-5; G1/99, A/Hong Kong/1073/1999 (H9N2).