Protection following vaccination with PAV3-HA or AdHu5-HA vaccine.

<p>(A–C) Groups of 9 BALB/c mice were vaccinated with 10¹⁰ vp/mouse of PAV3-HA (▵), AdHu5-HA (▴), or PBS Control (•) and challenged with 100LD50 of H5N1-H05 on days 5, 8, and 10 post-vaccination. (A) Protection afforded 5-days post-vaccination. (i) survival and (ii) weight loss. (B) Protection afforded 8-days post-vaccination. (i) survival and (ii) weight loss. (C) Protection afforded 10-days post-vaccination. (i) survival and (ii) weight loss. The data represent average values and standard deviations from 2 experiments performed with 2 independent vector preparations of each vaccine (D) Lung viral titres 3 days after lethal challenge. Groups of 6 BALB/c were vaccinated with 10¹⁰ vp/mouse of PAV3-HA () or AdHu5-HA (), or no vaccine (Control, ()) and challenged with 100LD50 of H5N1-H05 on days 5, 8, and 10 post-vaccination. Lungs were harvested from the mice on day 3 post-challenge. Virus titre was determined by TCID50 assay using serial dilution of lung homogenates on MDCK cells and monitoring for the presence of CPE over 48 hours. The TCID50 titre was calculated by the Reed & Muench method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015301#pone.0015301-Reed1" target="_blank">[39]</a> and normalized/gram of lung tissue. Data is presented as log₁₀ TCID50/g of lung tissue. The data represent average values and standard deviations from one experiment performed with one vector preparation of each vaccine (* and ** represents p<0.05).