Effect of EGFR silencing on cell migration.

<p>HCC827 GR5-G (maintained in the absence of gefitinib for 10 days) were transfected with EGFR siRNA or control siRNA (scramble) for 48 h. Then medium was replaced with fresh medium with or without gefitinib 1μM for 16h and the expression of the indicated proteins was analyzed by Western blotting (A) or cells were seeded on culture inserts in the absence or in the presence of 1μM gefitinib for migration assay (B). Representative fields of migration are shown (magnification of 100X). Columns, means of 10 fields counted; bars, SD. Results are representative of three independent experiments. ***P<0.001.