Effect of EGF on migration of HepG2 cells.

<p>(A) Relative cell migration rate was determined using wound closure assay in HepG2 cells incubated in the absence or presence of 5, 10, 30, 50, and 100 ng/mL EGF for 24 h. (B) The cell migration was assessed by the Transwell migration assay in HepG2 cells incubated in the absence (control) or presence of 10 ng/mL EGF for 8 h. (C) HepG2 cells were cultured in the absence (control) or presence of 10 ng/mL EGF for 24 h and cell cycle was analyzed by flow cytometry. (D) HepG2 cells were cultured in the absence (control) or presence of 10 ng/mL EGF for 24 or 48 h and cell proliferation was analyzed by MTT assays. Each value represents the mean ± SD of 5 independent determinations. *: <i>P</i><0.05, referring to the difference between cells treated with and without EGF.