Effect of EGFR inhibition on cell migration and invasion of H1975 cells.

<p>(A) H1975 cells were treated with 1μM gefitinib, PD168393 or afatinib. After 5h protein expression was assessed by Western blotting using the indicated antibodies. (B) H1975 cells were seeded on culture inserts in the absence or in the presence of 1μM EGFR inhibitors for (B) migration and (D) invasion assay. (C) Wound-healing assay was performed on H1975 cells in the presence of 1μM EGFR inhibitors. Columns, means of 10 fields counted; bars, SD. Results are representative of three independent experiments. ***P<0.001, **P<0.01.