Effect of EGFt treatment on EGFR degradation.

<p>A MCF-7 and Caco-2 cycloheximide treated cells were incubated with 3 nM, 150 nM hEGF or 150 nM EGFt at 37°C for different time periods as indicated. Cells were lysed, and the amount of EGFR was determined by Western blotting. Actin levels were used as loading control. B Levels of EGFR were determined by densitometry and normalized versus actin levels. Each column represents the mean of two replicates ± SEM.