Egfr knockdown suppressed SC proliferation and migration.

<p>(<b>A-C</b>) Histograms compared the Egfr expression at mRNA and protein levels in primary SCs transfected with Egfr siRNA (si Egfr) and siRNA control (si Con), thus validating the efficacy of Egfr knockdown. Also shown <b>(B)</b> is representative Western blot images. GAPDH was used as an internal control. (<b>D</b>) Double staining with EdU (red) plus Hoechst 33342 (blue) showed that Egfr siRNA (si Egfr) inhibited SC proliferation compared to that of siRNA control (si Con). (<b>E</b>) Transwell-based migration assay showed that Egfr siRNA (si Egfr) inhibited SC migration compared to that of siRNA control (si Con). Also shown are the resulting histograms. **<i>P</i>< 0.01 versus siRNA control (si Con).