<div><p>HCC827 GR5 cells were deprived of gefitinib for 1, 2, 3, 4, 7, 15 or 30 days. (A) Migration assay was performed at each time point. Columns, means of 10 field counted; bars, SD. ***P<0.001, **P<0.01.</p> <p>(B) Expression of the indicated proteins was analyzed by Western blotting at each time point. Results are representative of three independent experiments. (C) HCC827 GR5 grown in the presence of 1 µM gefitinib and HCC827 GR5-G (maintained in the absence of gefitinib for 7 days) lysates were incubated with human phospho-kinase array membranes and bound phospho-proteins were detected according to kit instructions. Each membrane contains specific kinase and positive control antibodies (P) spotted in duplicate. Columns, means of rela...
<p>(A) Phosphorylation pattern of p42/44 MAPK (Erk1/2) in cells treated with gefitinib 5 µM and DIM-...
<p>(A) The effect of gefitinib on p-AKT, p-tyrosine and BIM levels in NSCLC cells. H1650 and HCC827 ...
<p><b>(a-b)</b> Simulations of time-dependent phosphorylation of STAT3 and ERK in response to Gefiti...
<div><p>(A) HCC827 GR5-G (maintained in the absence of gefitinib for 7 days) cells were exposed to d...
<p>HCC827 GR5-G (maintained in the absence of gefitinib for 10 days) were transfected with EGFR siRN...
<p>(A) HCC827 GR5 cells were deprived of gefitinib for 1, 7, 14, 21 or 30 days. Expression of the in...
<p>Wound-healing, migration, invasion, and soft agar colony assays were performed on HCC827 GR5 grow...
<p>(A) Bright-field microscopy images of the parental PC9/HCC827, gefitinib-resistant PC9 (PC9-GR)/H...
<p>(A) PC-9/wt, PC-9/gefB4 and PC-9/gefE3 cells were treated with gefitinib (nM) at various concentr...
PDAC cell lines were treated with 100 nM gefitinib, 10 μM gefitinib or vehicle control for 24 hours,...
<p>(A) Phospho-RTK (top) and phospho-kinase (bottom) array analyses performed on untreated parental ...
<p>(<b>A</b> - <b>D</b>) Autophagic flux assays performed in SKBR3 cells. (<b>A</b>) Western blot an...
<p>H460 cells were loaded with 1 μM Hoechst 33342 in the presence of the indicated concentrations of...
<p>Time course of 10 nM [<sup>3</sup>H]gefitinib (A) and 1 μM Hoechst 33342 (B) accumulation in H460...
Total EGFR and total ERK are also shown for comparison. β-actin western blots and Coomassie blue sta...
<p>(A) Phosphorylation pattern of p42/44 MAPK (Erk1/2) in cells treated with gefitinib 5 µM and DIM-...
<p>(A) The effect of gefitinib on p-AKT, p-tyrosine and BIM levels in NSCLC cells. H1650 and HCC827 ...
<p><b>(a-b)</b> Simulations of time-dependent phosphorylation of STAT3 and ERK in response to Gefiti...
<div><p>(A) HCC827 GR5-G (maintained in the absence of gefitinib for 7 days) cells were exposed to d...
<p>HCC827 GR5-G (maintained in the absence of gefitinib for 10 days) were transfected with EGFR siRN...
<p>(A) HCC827 GR5 cells were deprived of gefitinib for 1, 7, 14, 21 or 30 days. Expression of the in...
<p>Wound-healing, migration, invasion, and soft agar colony assays were performed on HCC827 GR5 grow...
<p>(A) Bright-field microscopy images of the parental PC9/HCC827, gefitinib-resistant PC9 (PC9-GR)/H...
<p>(A) PC-9/wt, PC-9/gefB4 and PC-9/gefE3 cells were treated with gefitinib (nM) at various concentr...
PDAC cell lines were treated with 100 nM gefitinib, 10 μM gefitinib or vehicle control for 24 hours,...
<p>(A) Phospho-RTK (top) and phospho-kinase (bottom) array analyses performed on untreated parental ...
<p>(<b>A</b> - <b>D</b>) Autophagic flux assays performed in SKBR3 cells. (<b>A</b>) Western blot an...
<p>H460 cells were loaded with 1 μM Hoechst 33342 in the presence of the indicated concentrations of...
<p>Time course of 10 nM [<sup>3</sup>H]gefitinib (A) and 1 μM Hoechst 33342 (B) accumulation in H460...
Total EGFR and total ERK are also shown for comparison. β-actin western blots and Coomassie blue sta...
<p>(A) Phosphorylation pattern of p42/44 MAPK (Erk1/2) in cells treated with gefitinib 5 µM and DIM-...
<p>(A) The effect of gefitinib on p-AKT, p-tyrosine and BIM levels in NSCLC cells. H1650 and HCC827 ...
<p><b>(a-b)</b> Simulations of time-dependent phosphorylation of STAT3 and ERK in response to Gefiti...