Cyclin D1 expression and pRB phosphorylation was inhibited while PAX6 expression was suppressed.

<p>A, B, The expression of cyclin D1, pRB and the phosphorylated pRB in A549 PAX6 KD, A549 NC, A549 cells as well as H1299 PAX6KD, H1299NC, H1299 cells was determined by Western blotting. β-actin and GAPDH expression level was measured as internal loading controls respectively. Cyclin D1 and pRB levels were measured by the gray level and were normalized by internal loading controls. Data are expressed as mean ±SEM. Times of the experiments are listed above the histograms. *P <0.05, **P <0.01.